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ATCC
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Thermo Fisher
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Thermo Fisher
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ATCC
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ATCC
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ATCC
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Image Search Results
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: The MHC region on the short arm of human chromosome 6, contains the HLA-DR (HLA-DRA and HLA-DRB) molecules. The allelic variants of HLA-DRB1 ( DRB1 *04, *13, and *15) are linked with only one of the genes HLA-DRB4 , or HLA-DRB3 , or HLA-DRB5 .
Article Snippet: Primary antibodies were diluted in 5%
Techniques:
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: A) Heat map of differentially expressed genes (log transformed normalized gene counts) in PBMCs of HLA-DRB1 SE-positive [*04 ( n = 17)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. Genes shown in red have higher gene counts and those shown in blue have lower gene counts. B) HLA-DRB1 gene expression in PBMCs of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. C) HLA-DRB1 gene expression in PBMCs of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 6)] and SE-negative [*03:01/*15:01 ( n = 6)] individuals. FC: fold change, ns: non-significant adjusted P -value.
Article Snippet: Primary antibodies were diluted in 5%
Techniques: Transformation Assay, Expressing
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: A) Heat map of differentially expressed genes (log transformed normalized gene counts) in CD4+ T cells of HLA-DRB1 SE-positive [*04 ( n = 14)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 12)] individuals. B) Heatmap of differentially expressed genes (log transformed normalized gene counts) in CD8+ T cells of HLA-DRB1 SE-positive [*04 ( n = 13)] versus SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 8)] individuals. In both heatmaps, genes shown in red have higher gene counts and those shown in blue have lower gene counts. C) HLA-DRB1 gene expression in CD4+ T cells of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 4)] and SE-negative [*03:01/*15:01 ( n = 5)] individuals. D) HLA-DRB1 gene expression in CD8+ T cells of HLA-DRB1 SE-positive [*03:01/*04:01 ( n = 3)] and SE-negative [*03:01/*15:01 ( n = 4)] individuals. FC: fold change, ns: non-significant adjusted P -value.
Article Snippet: Primary antibodies were diluted in 5%
Techniques: Transformation Assay, Expressing
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: A) Heat map of differentially expressed genes (log transformed normalized gene counts) in CD14+ monocytes of HLA-DRB1 SE-positive [*04 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 4)] individuals. Genes shown in red have higher gene counts and those shown in blue have lower gene counts for the contrast SE+ vs. SE-. B) HLA-DRB1 gene expression in CD14+ monocytes of HLA-DRB1 SE-positive [*04 ( n = 6)] and SE-negative [*03:01/*13:01 and *03:01/*15:01 ( n = 4)] individuals. FC: fold change, ns: non-significant adjusted P -value.
Article Snippet: Primary antibodies were diluted in 5%
Techniques: Transformation Assay, Expressing
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: A) HLA-DRB1 gene expression in whole blood samples of HLA-DRB1 SE-positive [*04 ( n = 25)] and SE-negative [*03, *07, and *15 ( n = 44)] individuals. B) HLA-DRB1 gene expression in whole blood samples of individuals carrying different combinations of HLA-DRB1 alleles [*03/*03 ( n = 6), *03/*04 ( n = 5), *03/*07 ( n = 7), *03/*15 ( n = 17), *04/*04 ( n = 7), *04/*07 ( n = 6), *04/*15 ( n = 7), *07/*15 ( n = 13), and *15/*15 ( n = 1)]. *: Adjusted P -value (FDR) < 0.05.
Article Snippet: Primary antibodies were diluted in 5%
Techniques: Expressing
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: A) HLA-DRB1 gene expression in whole blood samples of HLA-DRB1 SE-positive [*04 ( n = 44)] and SE-negative [*03, *07, and *15 ( n = 21)] RA patients. B) HLA-DRB1 gene expression in whole blood samples of RA patients carrying different combinations of HLA-DRB1 alleles [*03/*03 ( n = 4), *03/*04 ( n = 7), *03/*07 ( n = 3), *03/*15 ( n = 6), *04/*04 ( n = 12), *04/*07 ( n = 7), *04/*15 ( n = 18), *07/*15 ( n = 2), and *15/*15 ( n = 6)]. *: Adjusted P -value (FDR) < 0.05.
Article Snippet: Primary antibodies were diluted in 5%
Techniques: Expressing
Journal: medRxiv
Article Title: Expression levels of HLA-DRB and HLA-DQ are associated with MHC Class II haplotypes in healthy individuals and rheumatoid arthritis patients
doi: 10.1101/2021.01.06.20249032
Figure Lengend Snippet: A) Representative western blots showing differential binding of different HLA-DRB antibodies (ab133578, DA2, ab202343, ab98108, and ab168534) to the recombinant proteins HLA-DRB1*03:01, HLA-DRB1*04:01, HLA-DRB1*15:01, and HLA-DRB5*01:01. B) Representative western blot of determining HLA-DRB levels using the antibody DA2 in PBMC samples of individuals carrying HLA-DRB1 *03:01/*04:01 versus HLA-DRB1 *03:01/*15:01 alleles. Vinculin was used as a protein loading control. C) Standard curve of the recombinant protein HLA-DRB1*04:01 detected by the antibody DA2. D) Standard curve of the recombinant protein HLA-DRB1*15:01 detected by the antibody DA2. E) Interpolated HLA-DRB protein levels in individuals carrying HLA-DRB1 *03:01/*04:01 and HLA-DRB1 *03:01/15:01 alleles.
Article Snippet: Primary antibodies were diluted in 5%
Techniques: Western Blot, Binding Assay, Recombinant